Biacore-Type Dual-Channel SPR Optical Engine
Kretschmann prism geometry with high-resolution 780 nm diode laser and linear CCD array.
Biomedical Engineering / SPR Immunosensor Kinetics
FREE TO EXPLORELABORATORY 15 · BIOMEDICAL ENGINEERING
Surface Plasmon Resonance (SPR) Biosensor Angle Shift & Biomolecular Affinity
Quantify the resonance angle shift of a gold-film SPR immunosensor as a function of analyte protein concentration to determine association kinetics and Langmuir adsorption linearity. Explore interactive physiological instrumentation simulation, biomechanics, and biomedical engineering telemetry.
BIOINSTRUMENTATION · BIOSIGNALS · BIOMECHANICS · IMAGING
Your virtual biomedical engineering laboratory.
RESONANCE ANGLE SHIFT ΔΘ—
PHYSIOLOGICAL & TEST CELL TELEMETRY
Ready to sample with Antigen C = 15.0 nM.
Adjust Antigen C, then execute biomedical trial.
Biomedical engineering telemetry and physiological states are evaluated rigorously using 64-bit IEEE 754 precision. Residuals reflect ordinary least-squares regression across successive observations. Continuous data structures support high-fidelity biological and physiological modeling.
Biomedical assumptions and boundary condition limits
The linear sensitivity model applies in the low-concentration sub-saturation regime (C_ag ≤ 30 nM). Evaluated using a 50 nm gold sensor chip functionalized with covalently coupled monoclonal anti-human IgG via an alkanethiol self-assembled monolayer (SAM). Analyte is human IgG diluted in HBS-EP running buffer (pH 7.4) perfused at 30 μL/min.
Biomedical apparatus & physiological sensors.
50 nm Sputtered Gold Sensor Chip (CM5 carboxymethylated)
Provides plasmonic gold surface with covalently immobilized anti-human IgG capture antibodies.
Integrated Microfluidic Cartridge (IFC)
Maintains continuous laminar flow (30 μL/min) across sample and reference flowcells.
Precision Temperature-Controlled Optical Deck (25.0 °C)
Eliminates refractive index thermal drift with ±0.01 °C Peltier thermal control.
Certified Purified Human IgG Target Antigen Stock
NIST-traceable protein standards prepared in HBS-EP running buffer (10 to 60 nM).
Sensorgram Real-Time Kinetic Telemetry Software
Tracks angle dip minimum with 0.1 millidegree (0.1 Resonance Unit, RU) resolution.
FOLLOW THE METHOD
A reason behind every step.
Quantify the resonance angle shift of a gold-film SPR immunosensor as a function of analyte protein concentration to determine association kinetics and Langmuir adsorption linearity. Standardized parameter settings: 5.0, 10.0, 15.0, 20.0, 25.0, 30.0 nM.
01
Sensor Chip Docking and Buffer Baseline Stabilization
Dock the functionalized CM5 gold chip into the optical block and prime flow channels with HBS-EP running buffer at 30 μL/min until baseline drift is < 0.2 mdeg/min.
02
Reference Flowcell Subtraction Setup
Configure Channel 1 as non-specific reference (mock-immobilized) and Channel 2 as active antibody surface for inline differential subtraction.
03
Initial Antigen Injection (5.0 nM)
Inject 5.0 nM human IgG antigen for 180 seconds, allowing binding association to reach equilibrium plateau; log net resonance angle shift Δθ in millidegrees.
04
Surface Regeneration Cycle
Inject a 30-second pulse of 10 mM glycine-HCl (pH 2.0) to dissociate bound antigen and regenerate baseline antibody binding capacity.
05
Concentration Progression Sequence
Sequentially inject antigen standards of 10.0, 15.0, 20.0, 25.0, and 30.0 nM, executing regeneration and baseline re-referencing at each step.
06
Sensitivity Regression and Affinity Analysis
Plot steady-state angle shift Δθ against concentration C_ag, performing linear regression to evaluate SPR refractive sensitivity k_spr.
YOUR NOTEBOOK · 0 OBSERVATIONS
Turn measurements into biomedical engineering insight.
Points are your recorded observations. The solid regression line validates SPR Immunosensor Kinetics mathematical scaling.
LINEAR REGRESSION FIT · y = mx + b
Δθ = k_spr · C_ag = 8.4000 · C_ag
| # | Antigen Concentration C_ag (nM) | Resonance Angle Shift Δθ (mdeg) | Antigen concentration C_ag (nM) | Resonance angle shift Δθ (millidegrees) | Remove |
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