Thermostatically Controlled Orbital Shaker Incubator (37.0 °C)
Maintains physiological temperature and gentle agitation (50 RPM) simulating in-vivo fluid flow.
Biomedical Engineering / Hydrogel Scaffold Degradation
FREE TO EXPLORELABORATORY 22 · BIOMEDICAL ENGINEERING
Biodegradable Hydrogel Scaffold Hydrolytic Degradation Kinetics
Quantify the hydrolytic degradation rate constant k_d and mass loss kinetics of a poly(lactic-co-glycolic acid) / PEG hydrogel tissue engineering scaffold in PBS at 37.0 °C. Explore interactive physiological instrumentation simulation, biomechanics, and biomedical engineering telemetry.
BIOINSTRUMENTATION · BIOSIGNALS · BIOMECHANICS · IMAGING
Your virtual biomedical engineering laboratory.
RESIDUAL DRY MASS PERCENTAGE M_RES—
PHYSIOLOGICAL & TEST CELL TELEMETRY
Ready to sample with Time t = 6.0 days.
Adjust Time t, then execute biomedical trial.
Biomedical engineering telemetry and physiological states are evaluated rigorously using 64-bit IEEE 754 precision. Residuals reflect ordinary least-squares regression across successive observations. Continuous data structures support high-fidelity biological and physiological modeling.
Biomedical assumptions and boundary condition limits
The linear zero-order degradation model applies to the initial mass loss phase (first 14 days); late-stage fragmentation exhibits non-linear mass loss. Evaluated on cylindrical 3D porous PLGA-PEG composite hydrogel scaffolds (porosity 85%, diameter 8.0 mm, height 4.0 mm, initial dry mass 50.0 mg) incubated in 10 mL PBS (pH 7.4) at 37 °C under gentle shaking. Linear hydrolytic degradation rate k_d = 3.2000 %/day across days 2 to 12.
Biomedical apparatus & physiological sensors.
Precision Analytical Balance (0.01 mg resolution)
Determines scaffold dry mass before and after degradation incubation intervals.
Laboratory Freeze Dryer / Lyophilizer (-50 °C, 0.05 mbar)
Sublimates all absorbed water to measure true residual polymer dry mass without collapsing pores.
Calibrated Digital Micro-pH Electrode
Monitors acidic lactic and glycolic acid by-product accumulation in the PBS incubation medium.
Milli-Q Ultra-Pure Water Washing Station
Rinses soluble buffer salts from degraded scaffolds prior to freeze drying.
Vacuum Desiccator with Silica Gel Indication
Prevents ambient moisture reabsorption during post-lyophilization mass determination.
FOLLOW THE METHOD
A reason behind every step.
Quantify the hydrolytic degradation rate constant k_d and mass loss kinetics of a poly(lactic-co-glycolic acid) / PEG hydrogel tissue engineering scaffold in PBS at 37.0 °C. Standardized parameter settings: 2.0, 4.0, 6.0, 8.0, 10.0, 12.0 days.
01
Scaffold Fabrication and Initial Dry Mass Baseline
Fabricate porous PLGA-PEG scaffolds via solvent casting/particulate leaching, lyophilize for 24 hours, and record initial dry mass W_0 (~50.00 mg) on analytical balance.
02
Sterile Incubation Media Preparation
Prepare 10 mL sterile phosphate buffered saline (PBS, pH 7.40) aliquots in sealed centrifuge tubes and pre-warm to 37.0 °C in orbital incubator.
03
Incubation and Dynamic Agitation
Submerge individual scaffolds into PBS tubes and incubate at 37.0 °C with continuous orbital shaking at 50 RPM.
04
Periodic Destructive Sampling Harvest
At incubation intervals of 2, 4, 6, 8, 10, and 12 days, harvest duplicate scaffold specimens and record media pH to detect acidic buildup.
05
Buffer Salt Rinsing and Lyophilization
Gently rinse harvested scaffolds three times in deionized water to remove crystallized buffer salts, freeze at -80 °C, and lyophilize for 48 hours to complete dryness.
06
Residual Mass Kinetics Regression
Weigh residual dry mass W_t, calculate residual percentage M_res = (W_t / W_0) · 100%, plot M_res versus time t, and evaluate degradation rate k_d.
YOUR NOTEBOOK · 0 OBSERVATIONS
Turn measurements into biomedical engineering insight.
Points are your recorded observations. The solid regression line validates Hydrogel Scaffold Degradation mathematical scaling.
LINEAR REGRESSION FIT · y = mx + b
M_res = -k_d · t + M_0 = -3.2000 · t + 98.0000
| # | Incubation Time t (days) | Residual Dry Mass Percentage M_res (%) | Incubation time t (days) | Residual dry mass M_res (%) | Remove |
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