Clark Polarographic Oxygen Electrode Probe
Platinum micro-cathode (25 μm) and concentric Ag/AgCl reference anode behind Teflon membrane.
Biomedical Engineering / Clark Oxygen Electrode
FREE TO EXPLORELABORATORY 06 · BIOMEDICAL ENGINEERING
Clark Polarographic Electrode Limiting Diffusion Current & Oxygen Tension
Measure the steady-state cathodic reduction limiting diffusion current of a membrane-covered Clark polarographic oxygen electrode across calibrated dissolved oxygen partial pressures. Explore interactive physiological instrumentation simulation, biomechanics, and biomedical engineering telemetry.
BIOINSTRUMENTATION · BIOSIGNALS · BIOMECHANICS · IMAGING
Your virtual biomedical engineering laboratory.
CATHODIC REDUCTION CURRENT I_D—
PHYSIOLOGICAL & TEST CELL TELEMETRY
Ready to sample with P_O2 = 8.0 kPa.
Adjust P_O2, then execute biomedical trial.
Biomedical engineering telemetry and physiological states are evaluated rigorously using 64-bit IEEE 754 precision. Residuals reflect ordinary least-squares regression across successive observations. Continuous data structures support high-fidelity biological and physiological modeling.
Biomedical assumptions and boundary condition limits
The linear diffusion model applies across physiological blood gas oxygen ranges (2.0 to 17.0 kPa). Evaluated on a 25 μm diameter platinum micro-cathode with 12 μm Teflon membrane in a 37.0 °C thermostatted micro-flowcell with -0.70 V polarizing potential. Calibrated across 2.0 kPa to 17.0 kPa (15 to 128 mmHg) oxygen partial pressure.
Biomedical apparatus & physiological sensors.
Thermostatted Water-Jacketed Micro-Flowcell
Maintains blood gas calibration temperature at strictly 37.00 ± 0.05 °C.
Precision Potentiostat Polarographic Controller
Applies regulated -0.700 V cathodic polarizing voltage and buffers nanoamp currents.
Calibrated Gas Tonometer Equilibration System
Equilibrates physiological saline with certified precision O2/N2/CO2 gas mixtures.
Femtoammeter / Low-Current Electrometer
Digitizes steady-state cathodic reduction current with 0.01 nA measurement precision.
NIST-Traceable Barometer & Humidity Sensor
Calculates true dry gas partial pressures accounting for water vapor saturation.
FOLLOW THE METHOD
A reason behind every step.
Measure the steady-state cathodic reduction limiting diffusion current of a membrane-covered Clark polarographic oxygen electrode across calibrated dissolved oxygen partial pressures. Standardized parameter settings: 2.0, 5.0, 8.0, 11.0, 14.0, 17.0 kPa.
01
Membrane Inspection and Polarizing Setup
Inspect the 12 μm Teflon membrane for micro-tears, fill electrolyte well with fresh 0.1 M KCl buffer, and apply -0.700 V cathodic polarizing potential.
02
Zero-Oxygen Baseline Calibration
Perfuse the flowcell with zero-oxygen calibration fluid (deoxygenated with sodium sulfite) and record residual dark background current (I_0 < 0.1 nA).
03
Initial Oxygen Step Calibration
Perfuse saline tonometered to P_O2 = 2.0 kPa (15.0 mmHg) at 37.0 °C, establishing a constant perfusion velocity to prevent boundary layer depletion.
04
Diffusion Plateau Current Acquisition
Allow 45 seconds for diffusion boundary layer stabilization; record steady-state cathodic current I_d in nanoamperes.
05
Oxygen Partial Pressure Progression
Increment tonometered oxygen partial pressure through 5.0, 8.0, 11.0, 14.0, and 17.0 kPa (up to physiological normoxia ~100-128 mmHg), logging I_d at each step.
06
Diffusion Sensitivity Regression Analysis
Plot reduction current I_d against oxygen partial pressure P_O2, performing linear regression through zero to calculate diffusion sensitivity k_O2.
YOUR NOTEBOOK · 0 OBSERVATIONS
Turn measurements into biomedical engineering insight.
Points are your recorded observations. The solid regression line validates Clark Oxygen Electrode mathematical scaling.
LINEAR REGRESSION FIT · y = mx + b
I_d = k_O2 · P_O2 = 1.6500 · P_O2
| # | Oxygen Partial Pressure P_O2 (kPa) | Cathodic Reduction Current I_d (nA) | Oxygen partial pressure P_O2 (kPa) | Cathodic current I_d (nA) | Remove |
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