Screen-Printed GOx Platinum Biosensor Strip
Working electrode with immobilized glucose oxidase and permselective polyurethane outer membrane.
Biomedical Engineering / Glucose Biosensor Amperometry
FREE TO EXPLORELABORATORY 14 · BIOMEDICAL ENGINEERING
Enzymatic Amperometric Glucose Biosensor Kinetics & Linearity
Evaluate the steady-state catalytic current of a glucose oxidase (GOx) platinum-carbon amperometric electrode across blood glucose concentrations to evaluate sensor sensitivity and linear dynamic range. Explore interactive physiological instrumentation simulation, biomechanics, and biomedical engineering telemetry.
BIOINSTRUMENTATION · BIOSIGNALS · BIOMECHANICS · IMAGING
Your virtual biomedical engineering laboratory.
CATALYTIC OXIDATION CURRENT I_A—
PHYSIOLOGICAL & TEST CELL TELEMETRY
Ready to sample with Glucose C = 6.0 mmol/L.
Adjust Glucose C, then execute biomedical trial.
Biomedical engineering telemetry and physiological states are evaluated rigorously using 64-bit IEEE 754 precision. Residuals reflect ordinary least-squares regression across successive observations. Continuous data structures support high-fidelity biological and physiological modeling.
Biomedical assumptions and boundary condition limits
The linear kinetic model applies across the clinical range (2.0 to 12.0 mmol/L); higher levels enter enzyme saturation. Evaluated using a screen-printed three-electrode sensor (platinum working electrode, Ag/AgCl reference, carbon counter) polarized at +0.650 V in PBS buffer (pH 7.4, 37.0 °C). Evaluated across clinical normo- and hyperglycemic concentrations (2.0 to 12.0 mmol/L).
Biomedical apparatus & physiological sensors.
Precision Analytical Electrochemical Potentiostat
Applies constant +0.650 V anodic polarizing potential and records microampere currents.
Stirred Water-Jacketed Electrochemical Cell
Maintains reaction buffer at 37.00 ± 0.05 °C with constant 250 RPM magnetic stirring.
Calibrated Micropipette Set (10-1000 μL)
Dispenses calibrated standard glucose stock solution aliquots into the reaction cell.
Certified Glucose Standard Reference Solutions
Traceable 100 mmol/L β-D-glucose stock solution in saturated benzoic acid preservative.
Low-Noise Electrometer Data Logging Console
Digitizes anodic oxidation current at 10 Hz with 1.0 nA measurement precision.
FOLLOW THE METHOD
A reason behind every step.
Evaluate the steady-state catalytic current of a glucose oxidase (GOx) platinum-carbon amperometric electrode across blood glucose concentrations to evaluate sensor sensitivity and linear dynamic range. Standardized parameter settings: 2.0, 4.0, 6.0, 8.0, 10.0, 12.0 mmol/L.
01
Electrochemical Cell Conditioning
Fill the glass cell with 10.0 mL of phosphate-buffered saline (PBS, pH 7.4) at 37.0 °C; insert the three-electrode sensor strip and start magnetic stirring at 250 RPM.
02
Polarization and Background Current Decay
Apply +0.650 V polarizing potential to the platinum working electrode; wait 10 minutes until background capacitive current stabilizes (< 0.05 μA).
03
Initial Glucose Step Injection (2.0 mmol/L)
Inject calibrated volume of glucose stock to achieve 2.0 mmol/L (36 mg/dL); allow 30 seconds for enzymatic steady-state current plateau.
04
Catalytic Current Logging
Record steady-state anodic oxidation current I_a in microamperes over a 15-second integration window.
05
Concentration Progression Sequence
Perform sequential spiked standard additions to achieve 4.0, 6.0, 8.0, 10.0, and 12.0 mmol/L (up to severe hyperglycemia ~216 mg/dL), logging I_a at each step.
06
Sensitivity Regression and Linearity Verification
Plot catalytic current I_a against glucose concentration C_glu, performing linear regression through zero to determine biosensor sensitivity S_glu.
YOUR NOTEBOOK · 0 OBSERVATIONS
Turn measurements into biomedical engineering insight.
Points are your recorded observations. The solid regression line validates Glucose Biosensor Amperometry mathematical scaling.
LINEAR REGRESSION FIT · y = mx + b
I_a = S_glu · C_glu = 0.7200 · C_glu
| # | Glucose Concentration C_glu (mmol/L) | Catalytic Oxidation Current I_a (μA) | Glucose concentration C_glu (mmol/L) | Catalytic current I_a (μA) | Remove |
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